Contributions to the OPT and modeling of amylolysis with REC enzymes
978-3-659-37846-1
3659378461
120
2013-05-02
54.90 €
eng
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In the present study, the main characteristics and functions of amylase enzymes are summarized. Furthermore the identification and characterization of a new amylase from Photobacterium halotolerans isolated from salt marsh of Tarquinia, Viterbo Italy is described. For a more efficient expression of this enzyme, the Pichia pastoris expression system is studied and optimized with a specific flow rate of methanol during the glycerol-methanol transition phase, using a semiconductor gas sensor for methanol detection from the exhaust air of the fermentor.
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