Bookcover of Serine Protease Inhibitors
Booktitle:

Serine Protease Inhibitors

A Biochemical study

LAP LAMBERT Academic Publishing (2012-01-04 )

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ISBN-13:

978-3-8473-2009-8

ISBN-10:
3847320092
EAN:
9783847320098
Book language:
English
Blurb/Shorttext:
Proteinase inhibitors (PIs) are small proteins that are quite common in nature. PIs are present in multiple forms in numerous tissues of animals and plants as well as in microorganisms. Serine proteinase inhibitors are widespread in the plant kingdom. The present study was at investigating the biochemical properties of protease Lavatera cashmeriane seeds. Four protease inhibitors LC-pi I, LC-pi II, LC-pi III and LC-pi IV were purified from the seeds of Lavatera cashmeriane by ammonium sulphate precipitation and ion-exchange chromatography on DEAE-cellulose column. All were strong inhibitors of trypsin, chymotrypsin and elastase. The molecular weight of LC-pi I, LC-pi II, LC-pi III, and LC-pi IV was found to be 20.89, 14.12, 16.78 and 7.94kDa respectively by gel filtration chromatography and 10, 14, 16 and 7kDa respectively by SDS-PAGE. The SDS-PAGE revealed that LC-pi I is constituted of two subunits of 10,000 Da each. The optimum temperature for the inhibitors was found to be 30˚C for all inhibitors. LC-pi I showed strong antibacterial activity against Klebsiella pnuemoniea, and Pseudomonas aeruginosa but was less active against E.coli.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Shajrul Amin, Syed Rakashanda
Number of pages:
96
Published on:
2012-01-04
Stock:
Available
Category:
Biochemistry, biophysics
Price:
447.86 HK$
Keywords:
Proteae inhibitors, proteases, serine protease inhibitors

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