Bookcover of Bacterial P450s and steroid transformations
Booktitle:

Bacterial P450s and steroid transformations

Bacterial cytochrome P450 enzymes and steroid transformation by rhodococcal strains

LAP LAMBERT Academic Publishing (2011-04-27 )

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ISBN-13:

978-3-8443-3077-9

ISBN-10:
3844330771
EAN:
9783844330779
Book language:
English
Blurb/Shorttext:
Four bacterial cytochrome P450 enzyme activities are described in terms of their potential biotechnological use for steroid transformations, by engineered 'Rhodococcus' strains, into commercially valuable compounds. Three selected rhodococcal P450s were characterized with microbiological, genetic, biochemical, spectroscopic and chromatographical methods. Two sterol C26- hydroxylases were demonstrated to be important for 3- hydroxysterol degradation. The role of the novel dextromethorphan N-demethylase in sterol metabolism was discussed. Furthermore, heterologous production of a mutated P450 enzyme, from 'Bacillus megaterium', was shown in an engineered 'Rhodococcus' strain. The activity of the mutated P450 was identified as 16β-hydroxylase of 4- androstene-3,17-dione, by ¹H and ¹³C NMR. The detailed characterization of the cytochrome P450 enzymes, described in this book, provides insight into the catabolic repertoire of selected bacterial strains, necessary for engineering of steroid catabolic pathways in order to produce pharmaceuticals efficiently.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Kamila du Plessis
Number of pages:
156
Published on:
2011-04-27
Stock:
Available
Category:
Microbiology
Price:
59.00 €
Keywords:
Rhodococcus, cytochrome P450 monooxygenases, P450, CYP, CYP125, CYP257A1, CYP102A1, bacterial transformations, Steroids, Sterols, rhodococcal steroid metabolism, biology, microbiology, Biochemistry, genetics, Cyprus, Microbiology

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