Bottom-up proteomics
978-613-2-59383-2
6132593837
68
2010-08-17
156.60 R$
eng
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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Bottom-up proteomics is a common method to identify proteins and characterize their amino acid sequences and post-translational modifications by proteolytic digestion of proteins prior to analysis by mass spectrometry.The proteins may first be purified by a method such as gel electrophoresis resulting in one or a few proteins in each proteolytic digest. Alternatively, the crude protein extract is digested directly, followed by one or more dimensions of separation of the peptides by liquid chromatography coupled to mass spectrometry.
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