9783659440540

Access to Prokaryotic Diversity of Unkeshwar Hot Spring, India

Bhagwan Rekadwad
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ISBN-13:

978-3-659-44054-0

ISBN-10:
365944054X
EAN:
9783659440540
Book language:
English
Blurb/Shorttext:
Total 46 thermophilic and thermoduric species were isolated at high temperatures from a hot spring in Unkeshwar, Nanded district, Maharashtra state, India (19o 85’ N and 78o 25’ E). All the isolates characterized using morphological and biochemical methods. All 46 isolates showed facultative growth 12-16 h of incubation at 70 °C. The bacterial strains could tolerate a temperature range between 45-90 °C (optimum 45–65 °C) and pH between 5-9 (optimum 7-8). Based on morphological characteristics and biochemical tests and 16S rRNA gene analysis, all isolates belonged to Firmicute followed by proteobacteria and followed by actinobacteria. Of 46 isolates, 17 morphologically diverse thermophilic bacteria have been further characterized and identification made in former step confirmed using 16S rRNA gene sequence analysis. To the best of our knowledge this is the first report of microbial diversity associated with Unkeshwar hot spring in Nanded district of Maharashtra State (India). Our results revealed presence of different thermophilic and thermoduric industrially important products stable at high temperatures such as amylase, gelatinase, protease, urease and oxidase and antibiotics.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Bhagwan Rekadwad
Number of pages:
148
Published on:
2015-03-06
Stock:
Available
Category:
Microbiology
Price:
55.90 €
Keywords:
amylase, Biocatalysts, Extremophiles, Microbial diversity, thermostability, Geobacillus, Ionic concentration

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